Bala was badly injured after an "atypical interaction" with his brother Bhutan, Rebecca Romzek, a public relations and communications specialist at the park, told CNN on Wednesday.
The tiger was transported to the animal care center at the ambulance park, but veterinarians failed to rescue it, the park reported Tuesday.
"Despite the best efforts of veterinarians, her wounds were too large and she was humanely euthanized," Romzek said. "A complete necropsy is currently underway and the team will know more about passing it once the test results are obtained."
Bala and Bhutan have lived together all their lives, and the type of behavior that led to Bala's death has never been demonstrated. , officials said. They were born at a Texas zoo and moved to Tampa to live in the Jungle Habitat in Bush Gardens when they opened in 2007.
This is the first fatal trauma between tigers in the park's history, Romsek said.
In December, Bush Gardens of Tampa Bay received a Certificate of Human Approval through the American Humanities Conservation Program for "demonstrating the well-being and treatment of creatures that care for it."
"Bala lives with her brother Bhutan in the park since 2007, receiving world-class care, "the park says on Facebook. "Known for its unique coloring, Bala was loved by her care team and the guests who visited the Jungle."