US Secretary of State Mike Pompeo denied that Huawei's CEO was detained in Canada as a negotiating market in the US-China trade war.
Meng Wenzhou, 47, was arrested in Canada in December at the request of the United States.
The United States wants her extradition to be tried on charges of fraud related to alleged violation of sanctions against Iran.
Her lawyers argue that the arrest is politically motivated, citing US President Donald Trump's own remarks.
The case has halted relations between China, Canada and the United States.
Mr Pompeo was asked on Thursday at a press conference in Ottawa if he saw Mrs Meng's detention as something that could be used by the US for trade talks.
"No," said a top diplomat in America, who was in the Canadian capital, to meet with Prime Minister Justin Trudeau and Foreign Minister Christia Freeland.
President Trump had previously speculated that he could interfere with the executive branch if it would help secure a trade deal with China.
"Anything good for this country, I would do," he said last December.
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Mr Trump recently threatened to impose additional tariffs on $ 300 billion (245 billion British pounds) of Chinese imports from next month, in addition to $ 250 billion in Chinese goods, which are already taxed at 25% of the US.
Beijing gave way, imposing duties on American products, including chemicals, coal and medical equipment.
The Chinese Embassy in Canada told on Thursday the bilateral ties between the two countries are suffering and called for the release of Huawei's executive branch.
"China-Canada relations are now experiencing great difficulties and the Canadian country knows the root cause very well," the embassy said in an email to Reuters.
Ms. Meng is currently under house arrest in Vancouver, Canada, and is fighting an extradition trial in the United States.
Canada has confirmed reports that it has halted travel to China for its staff at the Hong Kong Consulate, though not for its diplomats.
Global Affairs says in a statement to the BBC that local officials "will not undertake official business trips outside Hong Kong."
A local official at the British Consulate was detained in China during a business trip this month.
On Thursday, the US Secretary of State also offered assurances of support for the Trump administration for two Canadians detained in China.
Canada seeks the support of its global allies in the cases of Michael Kovrig and Michael Spavor, who are facing espionage charges.
The two men were detained in China shortly after Canada arrested Ms Meng on behalf of the United States. The duo has been accused of damaging national security.
Their arrests are widely regarded as a tit for pushing Canada to release Ms. Meng.
Mr Pompeo stated that the United States would continue to raise the issue of "illegally detaining the two Canadians" with China "so far as they are home and returning to their families."
He also rejected any comparison of Ms. Meng's case with Mr. Spavor's and Mr. Kovrig's.
China, he told a journalist, wants "to talk about these two as if they were equal, as if they were morally similar, which is not their basis."